La prueba se basa en demostrar si la bacteria es capaz de hidrolizar a la esculina. Si esto ocurre la esculina se descompone en esculetina y glucosa. Por su parte, la bilis es un inhibidor que impide el crecimiento de algunos microorganismos, por tanto, la bacteria antes de desdoblar la esculina tiene que ser capaz de crecer en presencia de la bilis. Por ello, este medio se considera selectivo.

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Final pH 6. Store tubed media Cat. Media should not be used if there are any signs of deterioration shrinking, cracking, or discoloration , contamination, or if the expiration date has passed. Product is light and temperature sensitive; protect from light, excessive heat and freezing.

It should be used only with cultures of isolated organism. This product is used in conjunction with other biochemical tests to identify cultures of isolated organism. Inoculate and streak the medium with one isolated pure colony. Observe for growth and blackening of the medium. Consult listed references for the identification of colony morphology and further biochemical tests required for identification.

A heavy inoculum on BEA may cause interpretation of the bile esculin test difficult to read. Excess inoculum decreases the ability of the bile to inhibit growth of other gram-positive organisms that may hydrolyze esculin. There are a few streptococci that do not hydrolyze esculin but will grow in the presence of bile. Growth without blackening of this medium does not constitute a positive test.

BEA does not contain azide; as a result, gram-negative rods will grow on this medium. Many of these organisms may hydrolyze esculin.

IR2101 PDF

Agar bilis esculina: fundamento, preparaciĆ³n y usos



Bile esculin agar


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